PCM1 Antibody

• Catalog Number: orb1263190
• Category: Antibodies
• Description: PCM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 632-661 amino acids from the Central region of human PCM-1.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 229 kDa
• Target: PCM1
• UniProt ID: Q15154
• NCBI: Q15154
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Pericentriolar material 1 protein, PCM-1, hPCM-1,
• Note: For research use only
• Application notes: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Fluorescent image of A549 cell stained with PCM-1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.

Western blot analysis of PCM-1 antibody in Ramos cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PCM-1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.