PCK1 Antibody

• Catalog Number: orb1247136
• Category: Antibodies
• Description: PCK1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HVNWFRKDKEGK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:Paraffin embedded Human Cerebral Cortex, Kidney and Liver. Recommended concentration: 2ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PCK1
• UniProt ID: P35558
• NCBI: NP_002582.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PCK1, phosphoenolpyruvate carboxykinase 1 (soluble
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:Paraffin embedded Human Cerebral Cortex, Kidney and Liver. Recommended concentration: 2ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247136 (2 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247136 (2 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247136 (2 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.