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Catalog Number | orb526999 |
---|---|
Category | Antibodies |
Description | PCDH15 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC, IHC-Fr, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence of human PCDH15 (DLTVYAIDPQTNRAIDRNELFKFLDGKLLDINKDFQ). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 190 kDa |
UniProt ID | Q96QU1 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Protocadherin-15; PCDH15; USH1F Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U-87MG cells using anti-PCDH15 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis anti-PCDH15 antibody.Lane 1:human placenta Tissue;2:human U2OS Cell;3:human HeLa Cell;4:rat brain Tissue;5:rat lung Tissue;6:mouse brain Tissue;7:mouse lung Tissue;8:mouse kidney Tissue;9:mouse spleen Tissue;10:mouse Neuro-2a Cell.
IHC analysis of PCDH15 using anti-PCDH15 antibody.PCDH15 was detected in paraffin-embedded section of human skeletal muscle tissues.
IHC analysis of PCDH15 using anti-PCDH15 antibody.PCDH15 was detected in paraffin-embedded section of human prostatic cancer tissues.
IHC analysis of PCDH15 using anti-PCDH15 antibody.PCDH15 was detected in paraffin-embedded section of human rectal cancer tissues.
IHC analysis of PCDH15 using anti-PCDH15 antibody.PCDH15 was detected in paraffin-embedded section of human tonsil tissues.
IHC analysis of PCDH15 using anti-PCDH15 antibody.PCDH15 was detected in paraffin-embedded section of mouse brain tissues.
IHC analysis of PCDH15 using anti-PCDH15 antibody.PCDH15 was detected in paraffin-embedded section of mouse spleen tissues.
IHC analysis of PCDH15 using anti-PCDH15 antibody.PCDH15 was detected in paraffin-embedded section of rat brain tissues.
IHC analysis of PCDH15 using anti-PCDH15 antibody.PCDH15 was detected in paraffin-embedded section of rat spleen tissues.
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