PCAF Antibody

• Catalog Number: orb1527994
• Category: Antibodies
• Description: PCAF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IP, WB
• Reactivity: Human
• Immunogen: between 782 and 832
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PCAF
• UniProt ID: Q92831
• NCBI: NP_003875.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: K(lysine) acetyltransferase 2B; p300/CBP-associate
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse PCAF by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCAF antibody orb1527994 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PCAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCAF antibody orb1527994 used for IP at 6 µg per reaction.

Localization of PCAF Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-PCAF antibody orb1527994 and analyzed by DNA sequencing. The figure illustrates the peak distribution of PCAF binding within a 650 Kb region of chromosome 1 as detected using anti-PCAF antibody orb1527994.