Pc2 Antibody

• Catalog Number: orb1526769
• Category: Antibodies
• Description: Pc2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 150 and 200
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Pc2
• UniProt ID: O00257
• NCBI: NP_003646.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: NS5ATP1-binding protein 16; chromobox homolog 4 (P
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Pc2 by western blot. Samples: Whole cell lysate (10 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pc2 antibody (orb1526769) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse Pc2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pc2 antibody (orb1526769) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human Pc2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pc2 antibody (orb1526769) used for IP at 6 µg per reaction.

Detection of human Pc2 by immunohistochemistry.Sample: FFPE section of human bladder carcinoma. Antibody: Affinity purified rabbit anti- Pc2 (orb1526769) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse Pc2 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- Pc2 (orb1526769) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB