PAX8 Antibody

SKU: orb1252194

Description

PAX8 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  WB: 0.5-1 µg/mLIF: 1-2 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIHC (FFPE): 1-2 µg/mL for 30 min at RTThe stated application concentrations are suggested starting points. Titration of the PAX8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: PAX8/1492
• Immunogen: A human recombinant fragment (aa 60-261) was used as the immunogen for the PAX8 antibody.
• Target: PAX8
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Paired box protein Pax-8, PAX8

IHC testing of FFPE human thyroid carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.

IHC testing of FFPE human urothelial carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.

IHC testing of FFPE human renal cell carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.

Western blot testing of 1) human partial recombinant protein and 2) human Raji cell lysate with PAX8 antibody (clone PAX8/1492). Predicted molecular weight of isoforms 1-5: 31, 35, 42, 43 and 48 kDa, respectively. PAX8 can also be observed at ~62 kDa.

SDS-PAGE Analysis of Purified, BSA-Free PAX8 Antibody (clone PAX8/1492). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

PAX8

UniProt

Q06710