PAX8 Antibody

• Catalog Number: orb1252194
• Category: Antibodies
• Description: PAX8 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PAX8/1492
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: A human recombinant fragment (aa 60-261) was used as the immunogen for the PAX8 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: WB: 0.5-1 ug/mlIF: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIHC (FFPE): 1-2 ug/ml for 30 min at RTThe stated application concentrations are suggested starting points. Titration of the PAX8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PAX8
• UniProt ID: Q06710
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Paired box protein Pax-8, PAX8
• Note: For research use only
• Application notes: WB: 0.5-1 ug/mlIF: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIHC (FFPE): 1-2 ug/ml for 30 min at RTThe stated application concentrations are suggested starting points. Titration of the PAX8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human thyroid carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.

IHC testing of FFPE human urothelial carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.

IHC testing of FFPE human renal cell carcinoma with PAX8 antibody (clone PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.

Western blot testing of 1) human partial recombinant protein and 2) human Raji cell lysate with PAX8 antibody (clone PAX8/1492). Predicted molecular weight of isoforms 1-5: 31, 35, 42, 43 and 48 kDa, respectively. PAX8 can also be observed at ~62 kDa.

SDS-PAGE Analysis of Purified, BSA-Free PAX8 Antibody (clone PAX8/1492). Confirmation of Integrity and Purity of the Antibody.