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PAX8 Antibody Cocktail

Catalog Number: orb2640254

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2640254
CategoryAntibodies
DescriptionPAX8 is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.
Species/HostMouse
ClonalityMonoclonal
Clone NumberPAX8/1491 + PAX8/1492
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG2b + Mouse IgG2a
ImmunogenA human recombinant fragment (aa 60-261) was used as the immunogen for the PAX8 antibody cocktail.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis PAX8 antibody cocktail is available for research use only.
UniProt IDQ06710
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the PAX-8 antibody cocktail should be determined by the researcher.
Expiration Date12 months from date of receipt.
PAX8 Antibody Cocktail

IHC testing of FFPE human renal cell carcinoma with PAX8 antibody cocktail (clone PAX8-1491 + PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 minutes.

PAX8 Antibody Cocktail

Western blot testing of 1) human partial recombinant protein and 2) human Raji cell lysate with PAX8 antibody cocktail (clone PAX8/1491 + PAX8/1492). Predicted molecular weight of isoforms 1-5: 31, 35, 42, 43 and 48 kDa, respectively. PAX8 can also be observed at ~62 kDa.

  • PAX8 Antibody Cocktail [orb386148]

    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg