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Catalog Number | orb2640254 |
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Category | Antibodies |
Description | PAX8 is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | PAX8/1491 + PAX8/1492 |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2b + Mouse IgG2a |
Immunogen | A human recombinant fragment (aa 60-261) was used as the immunogen for the PAX8 antibody cocktail. |
Antibody Type | Primary Antibody |
Dilution range | Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This PAX8 antibody cocktail is available for research use only. |
UniProt ID | Q06710 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the PAX-8 antibody cocktail should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human renal cell carcinoma with PAX8 antibody cocktail (clone PAX8-1491 + PAX8/1492). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 minutes.
Western blot testing of 1) human partial recombinant protein and 2) human Raji cell lysate with PAX8 antibody cocktail (clone PAX8/1491 + PAX8/1492). Predicted molecular weight of isoforms 1-5: 31, 35, 42, 43 and 48 kDa, respectively. PAX8 can also be observed at ~62 kDa.