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Catalog Number | orb1538072 |
---|---|
Category | Antibodies |
Description | PAX7 Antibody (aa167-216) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Synthetic peptide located between aa167-216 of human PAX7 (P23759, NP_002575). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Zebra finch, Chicken (100%); Xenopus, Salmon, Zebrafish (92%). |
Concentration | 0.5 mg/ml |
Dilution range | IHC, IHC-P (5 µg/ml), WB (0.05 µg/ml) |
Conjugation | Unconjugated |
Target | PAX7 |
Storage | Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles. |
Buffer/Preservatives | PBS, 0.09% sodium azide, 2% sucrose. PBS, 0.09% sodium azide, 2% sucrose |
Alternative names | PAX7, HUP1, Paired box 7, Paired box gene 7, Paire Read more... |
Note | For research use only |
Application notes | Further information: Immunohistochemistry: LS-B3490 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3490 was determined to be 5 ug/ml. |
Expiration Date | 12 months from date of receipt. |
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