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Catalog Number | orb1263581 |
---|---|
Category | Antibodies |
Description | PAX6-T373 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-P, WB |
Reactivity | Human, Rat |
Isotype | Rabbit Ig |
Immunogen | This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-380 amino acids from human PAX6. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 47 kDa |
Target | PAX6 |
UniProt ID | P26367 |
NCBI | P26367 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Paired box protein Pax-6, Aniridia type II protein Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of PAX6-T373 in Y79 cell line lysates (35 ug/lane)
Western blot analysis of PAX6 using rabbit polyclonal PAX6 Antibody (T373) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.
PAX6-T373 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of PAX6-T373 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).