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Catalog Number | orb420247 |
---|---|
Category | Antibodies |
Description | PAX6 antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 7H10.D3.D4.G5 |
Tested applications | ELISA, IF, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:75,000 - 1:150,000, IF: 1:100, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-PAX6 was purified from clarified ascites by Protein A chromatography. Cross-reactivity with PAX6 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | P26367 |
NCBI | NP_000271.1 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-pax6 antibody, Paired box protein Pax-6 Read more... |
Note | For research use only |
Application notes | Anti-PAX6 antibody has been tested by ELISA and Immunofluorescence and suitable for Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46.7 kDa in size corresponding to PAX6 by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
FC, IF, IHC-P, WB | |
Bovine, Mouse, Rat, Xenopus | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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