PAX3 antibody

• Catalog Number: orb18763
• Category: Antibodies
• Description: Goat polyclonal antibody to PAX3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Rat
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 4.5 μg/ml
• Conjugation: Unconjugated
• MW: 53.0; 24.1; 22.7; 53.5; 56.0; 44.8; 45.2; 53.3
• Target: PAX3
• Entrez: 5077
• Protein Sequence: TTLAGAVPRMM
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PAX3 antibody, anti WS1 antibody, anti CDHS a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Oesophagus shows nuclear staining of cells in the Squamous Epithelium. Recommended concentration, 3ug/ml.WB: Preliminary experiments gave an approx 30-35kDa band in Human Kidney lysates at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of approx. 24-58kDda according to NP_000429.2; NP_039230.1; NP_852122;.1 NP_852123.1; NP_852124.1; NP_852125.1; NP_852126.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Duodenum lysate using PAX3 antibody

Immunohistochemical staining of Human Esophagus using PAX3 antibody

Immunohistochemical staining of Human Esophagus using PAX3 antibody