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Catalog Number | orb413061 |
---|---|
Category | Antibodies |
Description | PARN Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, IHC-Fr, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human PARN recombinant protein (Position: M1-Y301). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 78 kDa |
UniProt ID | O95453 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Poly (A)-specific ribonuclease PARN; Deadenylating Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-PARN antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis using anti-PARN antibody.Lane 1:HeLa cell;2:human placenta tissue;3:COLO-320 cell;4:HepG2 cell;5:PANC-1 cell;6:SGC-7901 cell;7:MDA-MB-231 cell;8:rat kidney tissue;9:mouse heart tissue;10:mouse kidney tissue.
IF analysis of PARN using anti-PARN antibody.PARN was detected in immunocytochemical section of A549 cell.
IHC analysis of PARN using anti-PARN antibody.PARN was detected in paraffin-embedded section of human mammary cancer tissue.
IHC analysis of PARN using anti-PARN antibody.PARN was detected in paraffin-embedded section of mouse small intestine tissue.
IHC analysis of PARN using anti-PARN antibody.PARN was detected in paraffin-embedded section of rat small intestine tissue.
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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