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Catalog Number | orb1474883 |
---|---|
Category | Antibodies |
Description | PARK7/DJ1 Antibody (monoclonal, 4B10) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 4B10 |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | IgG2b |
Immunogen | E.coli-derived human PARK7 recombinant protein (Position: A2-D189). Human PARK7 shares 91% amino acid (aa) sequence identity with both mouse and rat PARK7. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 22 kDa |
UniProt ID | Q99497 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Expiration Date | 12 months from date of receipt. |
IHC analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody. PARK7/DJ1 was detected in a paraffin-embedded section of human endometrial carcinoma tissue.
IHC analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody. PARK7/DJ1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue.
IHC analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody. PARK7/DJ1 was detected in a paraffin-embedded section of human liver cancer tissue.
IF analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody. PARK7/DJ1 was detected in an immunocytochemical section of HeLa cells.
Western blot analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody.
Flow Cytometry analysis of HeLa cells using anti-PARK7/DJ1 antibody(Blue line).Isotype control antibody(Green line) was mouse IgG.Unlabelled sample(Red line) was also used as a control.
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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