PARD6A Antibody

• Catalog Number: orb1247146
• Category: Antibodies
• Description: PARD6A Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-GSRIRGDGSGFSL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4kDa according to NP_058644.1) . Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10ug/ml. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverse section. Recommended concentration: 5-10ug/ml.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PARD6A
• UniProt ID: Q9NPB6
• NCBI: NP_001032358.1, NP_058644.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, p
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 40kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4kDa according to NP_058644.1) . Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10ug/ml. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverse section. Recommended concentration: 5-10ug/ml.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247146 (10 ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1247146 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247146 (2 ug/ml) staining of Jurkat (A) and U251 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247146 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).