PAR2 Antibody

• Catalog Number: orb1906377
• Category: Antibodies
• Description: PAR2 Antibody
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: Ab4999 (Par-B)
• Tested applications: ELISA
• Reactivity: Human, Mouse, Primate, Rat
• Isotype: IgG4
• Immunogen: The original antibody was generated using phage display techniques to pan for antibodies (Fab fragments) capable of immunospecifically binding to residues spanning the protease cleavage site at the N-terminal of PAR-2. The affinity maturation of the selected parental clone Ab3777 resulted in generation of the antibody.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: PAR2
• UniProt ID: P55085
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: F2RL1; PAR-2; GPR11; Proteinase-activated receptor
• Note: For research use only
• Application notes: This antibody was generated by the affinity maturation of a parental clone Ab3777. This antibody exhibited concentration-dependent binding to human PAR-2 transfected HEK293 cells with EC50 values of 0.2 nM. ELISA experiments demonstrated specific binding of this antibody to PAR-2 peptide (coupled to Transferrin as carrier). This antibody did not show any binding affinity for PAR-4 peptide or PAR-1 peptide. This antibody showed potent inhibition of calcium release in the human lung epithelial cell line A549 stimulated with trypsin similar to the parental clone Ab3777. (US20110311553).
• Expiration Date: 12 months from date of receipt.