PAR1/Thrombin Receptor/F2R Antibody

• Catalog Number: orb623800
• Category: Antibodies
• Description: PAR1/Thrombin Receptor/F2R Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PAR1/Thrombin Receptor/F2R recombinant protein (Position: K130-T425).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 47 kDa
• UniProt ID: P25116
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Proteinase-activated receptor 1; PAR-1; Coagulatio
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HL-60 cells using anti-F2R antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

IHC analysis of F2R using anti-F2R antibody.

IHC analysis of F2R using anti-F2R antibody.

WB analysis using anti-F2R antibody.Lane 1:placenta tissue, Lane 2:U-87MG cell.