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Catalog Number | orb234352 |
---|---|
Category | Antibodies |
Description | PAPP A/PAPPA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human PAPP A recombinant protein (Position: R95-Q388). Human PAPP A shares 88% amino acid (aa) sequence identity with mouse PAPP A. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 180973 MW |
UniProt ID | Q13219 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Pappalysin-1;3.4.24.79;Insulin-like growth factor- Read more... |
Note | For research use only |
Application notes | WB: The detection limit for PAPP A is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
IHC(P) analysis of Human Placenta Tissue using Anti-PAPP A Picoband antibody.
Western blot analysis using Anti-PAPP A Picoband antibody.Lane 1:Human Placenta Tissue;2:HT1080 Cell;3:SKOV Cell;4:22RV1 Cell;5:SW620 Cell;6:MM231 Cell.
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