PANC-1 Whole Cell Lysate

SKU: orb348710

Description

PANC-1 Whole Cell Lysate

Images & Validation

• Application Notes:
  orb348710 has been tested by SDS-PAGE. Multi-purpose PANC-1 Whole Cell Lysate are especially prepared as positive control for multiple assays including western blot, immunoprecipitation (IP), capture ELISA or other assays requiring native protein sample. For separation by SDS-PAGE and subsequent western blot analysis, lysates should be diluted by user to desired concentration in SDS-PAGE buffer with 2-mercaptoethanol or dithiothreitol as the reducing agent and heated to 95° C for 5 minutes. Sample is ready for use in immunoprecipitation and ELISA experiments, conditions should be optimized by the user. Rockland recommends its TrueBlot IP reagents for immunoprecipitation experiments.

Key Properties

• Purity: PANC-1 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate, 1 µM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 4 mg/ml with 1X RIPA buffer.
• Source: Human
• Biological Origin: Human
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -70° C or COLDER. For extended storage, aliquot lysate to minimize freeze/thaw cycles.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: None. Stabilizer: None. 1X RIPA Buffer with HALT Protease and Phosphatase Inhibitors
• Concentration: 4.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

PANC1 Lysate, Cell Lysate, PANC-1 lysate

SDS PAGE Results of PANC-1 Whole Cell Lysate. Lane 1: PANC-1 Whole Cell Lysate Non-Reduced [10 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: PANC-1 Whole Cell Lysate Reduced [10 µg]. 4-20% Gel, Coomassie Stained.