pan Antibody

• Catalog Number: orb1252417
• Category: Antibodies
• Description: pan Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRTL/1077 + KRTH/1076
• Tested applications: FC, IF, IHC-P
• Reactivity: Human, Rat
• Isotype: IgGs
• Immunogen: Recombinant human KRT77 and KRT76 protein were used as the immunogen for the pan Cytokeratin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the pan Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: pan
• UniProt ID: pan
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the pan Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with pan Cytokeratin antibody cocktail (KRTL/1077 + KRTH/1076).

Formalin paraffin rat stomach stained with pan Cytokeratin antibody cocktail (KRTL/1077 + KRTH/1076).

Formalin-fixed, paraffin-embedded rat Oviduct with pan Cytokeratin antibody cocktail (KRTL/1077 + KRTH/1076).