PAM Antibody

• Catalog Number: orb1527709
• Category: Antibodies
• Description: PAM Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Immunogen: between 2425 and 2475
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PAM
• UniProt ID: O75592
• NCBI: NP_055872.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: pam/highwire/rpm-1 protein; E3 ubiquitin-protein l
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PAM by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PAM antibody orb1527709 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. PAM was also immunoprecipitated by rabbit anti-PAM antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).