PALLD antibody

• Catalog Number: orb20083
• Category: Antibodies
• Description: Goat polyclonal antibody to PALLD
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:2000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 122
• Target: Palladin (aa895-907)
• Entrez: 23022
• Protein Sequence: RDSGDENEPIQER
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PALLD antibody, anti palladin, cytoskeletal a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx. 125kDa band observed in Human Umbillical Cord lysates (calculated MW of122kDa according to NP_057165.3). Recommended concentration: 0.1-0.3 μg/ml. An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
• Expiration Date: 12 months from date of receipt.

orb20083 (0.1ug/ml) staining of Human Umbillical Cord lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.