PAH Antibody

• Catalog Number: orb402402
• Category: Antibodies
• Description: PAH Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1% and 88.4% amino acid (aa) sequence identity with mouse and rat PAH, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 51862 MW
• UniProt ID: P00439
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Phenylalanine-4-hydroxylase;PAH;1.14.16.1;Phe-4-mo
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-PAH antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of PAH using anti-PAH antibody.Lane 1:human liver tissue;2:rat liver tissue;3:mouse liver tissue.

IF analysis of PAH using anti-PAH antibody.PAH was detected in immunocytochemical section of HepG2 cell.

IHC analysis of PAH using anti-PAH antibody.PAH was detected in paraffin-embedded section of rat kidney tissue.

IHC analysis of PAH using anti-PAH antibody.PAH was detected in paraffin-embedded section of human renal cancer tissue.

IHC analysis of PAH using anti-PAH antibody.PAH was detected in paraffin-embedded section of mouse kidney tissue.