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Catalog Number | orb2292468 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant PAFAH1B1. |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG2a Kappa |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Immunogen | PAFAH1B1 (NP_000421, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Protein Sequence | MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDVNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSP |
Tested applications | ELISA, IF, WB |
Clone Number | 5A5 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
NCBI | NP_000421 |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged PAFAH1B1 is 0.03 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to PAFAH1B1 on HeLa cell. [antibody concentration 15 ug/ml]
Western Blot analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5. Lane 1: PAFAH1B1 transfected lysate (46.6 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (37.84 KDa).