PAF49 Antibody

• Catalog Number: orb1528666
• Category: Antibodies
• Description: PAF49 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: between 404 and 454
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; ICC-IF - 1:50 - 1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PAF49
• UniProt ID: O15446
• NCBI: NP_036231.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: CD3e molecule, epsilon associated protein; RNA pol
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PAF49 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PAF49 used at a dilution of 1:200 (1µg/ml).

Detection of human PAF49 by WB and IP.Samples: HeLa cells. Antibodies: Affinity purified rabbit anti-PAF49 antibody orb1528666 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate.

Detection of human PAF49 by ICC. Sample: HeLa cells. Antibody: Affinity purified rabbit anti-PAF49 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody.