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Catalog Number | orb1247150 |
---|---|
Category | Antibodies |
Description | PADI4 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: AQGTLIRVTPEQPC |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 74kDa band observed in Human Spleen lysates (calculated MW of 74.1kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx. 75kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PADI4 |
UniProt ID | Q9UM07 |
NCBI | NP_036519.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PADI4, PAD, PDI4, PDI5, PADI5, peptidyl arginine d Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 74kDa band observed in Human Spleen lysates (calculated MW of 74.1kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx. 75kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247150 (0.3 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
HEK293 overexpressing PADI4 and probed with orb1247150 (mock transfection in first lane).
orb1247150 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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