Paclitaxel

SKU: orb1225286

Description

Paclitaxel is a novel antineoplastic agent, which was discovered in a screen of extracts of thousands of plants and natural products for antineoplastic activity by a National Y Institute program. Alought it functions as a mitotic inhibitor like vinca alkaloids, paclitaxel promotes the polymerization of tubulin instead of inducing the disassembly of microtubules, which inhibits the microtubules disassembly and promotes the formation of excessively stable, dysfunctional microtubules. Paclitaxel has exhibited antitumor activity against a broad spectrum of human Ys, including ovarian, breast, head and neck, and lung Y, in a large number of studies.(In Vitro):Paclitaxel (20 nM; 48 hours) induces programmed cell death and exists a block at the G2/M phase of the cell cycle.Paclitaxel (20 nM; 48 hours) induces a consistent increase in the level of p53.(In Vivo):Paclitaxel (1-20 mg/kg; i.p.; 1 time/2 days for five cycles) obviously induces liver metastases at the low-Paclitaxel group with little influence on primary tumor growth.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 33069-62-4
• MW: 853.92
• Purity: >98% (HPLC)
• Formula: C₄₇H₅₁NO₁₄
• SMILES: O=C1[C@H](OC(C)=O)C(C2(C)C)=C(C)[C@@H](OC([C@H](O)[C@@H](NC(C3=CC=CC=C3)=O)C4=CC=CC=C4)=O)C[C@@]2(O)[C@@H](OC(C5=CC=CC=C5)=O)[C@@]6([H])[C@@]1(C)[C@@H](O)C[C@@]7([H])OC[C@]76OC(C)=O
• Target: Microtubule/Tubulin
• Solubility: Ethanol: 18 mg/mL (21.07 mM); DMSO: 171 mg/mL (200.25 mM)

Bioactivity

• In Vivo:
  Animal model: MDA-231 xenograft-bearing mice. Dosage: 1, 20 mg/kg. Administration: Intraperitoneal injection; five cycles (1 time/2 days). Result: Liver metastases were obviously induced in the low-PTX (1 mg/kg) group with little influence on primary tumor growth compared with high-PTX group.
• In Vitro:
  Apoptosis Analysis Cell line: MCF-7, MDA-MB-231 cells. Concentration: 20 nM. Incubation time: 48 hours. Result: Induced programmed cell death. Cell Cycle Analysis Cell line: MCF-7, MDA-MB-231 cells. Concentration: 20 nM. Incubation time: 48 hours. Result: > 60% of MCF-7 cells and 50% of MDA-MB-231 cells were in the G2/M phase following 24 h treament. Western blot analysis. Cell line: MCF-7 cells (harboring wild-type p53 ). Concentration: 20 nM. Incubation time: 48 hours. Result: Induced a consistent increase in the level of p53.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC 125973

Quick Database Links

Gene Symbol

Microtubule/Tubulin