p90 RSK1 (phospho-S732) antibody

• Catalog Number: orb345669
• Category: Antibodies
• Description: p90 RSK1 (phospho-S732) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
• Concentration: 1.0mg/mL
• Dilution range: ELISA: 1:5000-1:50,000, WB: 1:1000-1:3000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein phosphorylated at S732. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Partially cross reactivity may occur against opossum and chicken based on > 90% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q15418
• NCBI: NP_001006666.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-p90 RSK1 pS732 antibody, rabbit anti-R
• Note: For research use only
• Application notes: This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 pS732 protein is expected in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of (lane 2) HEK293T cell lysates using p90 RSK1 (phospho-S732) antibody