p65 (phospho-T60) antibody

• Catalog Number: orb304662
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p65 (phospho-T60)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T60 of human WNK1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: WNK1
• Entrez: 116477, 232341, 65125
• UniProt ID: P83741, Q9H4A3, Q9JIH7
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HSN2 antibody, anti KDP antibody, anti KIAA03
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Feng, Shandan et al. Resveratrol Plays Protective Roles on Kidney of Uremic Rats via Activating HSP70 Expression Biomed Res Int, 2020, 2126748 (2020)

PubMed: 32280682

Applications

WB

Reactivity

Rat

Yi, Lanjie et al. Formononetin inhibits inflammation and promotes gastric mucosal angiogenesis in gastric ulcer rats through regulating NF-κB signaling pathway J Recept Signal Transduct Res, (2020)

PubMed: 33100111

Applications

WB

Reactivity

Rat

Western blot analysis of WNK1 (Phospho-T60) expression in HEK293T EGF-treated (A), Jurkat EGF-treated (B) whole cell lysates. (Predicted band size: 250 kD; Observed band size: 230 kD)

Immunohistochemical analysis of WNK1 (Phospho-T60) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.