p600 Antibody

• Catalog Number: orb1526908
• Category: Antibodies
• Description: p600 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1350 and 1400
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2-10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: p600
• UniProt ID: Q5T4S7
• NCBI: NP_065816.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: E3 ubiquitin-protein ligase UBR4; zinc finger UBR1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse p600 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p600 antibody (orb1526908) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human p600 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Jurkat, HEK293T, RKO, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p600 antibody (orb1526908) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human p600 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p600 antibody (orb1526908) used for IP at 6 µg per reaction.

Detection of human p600 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-p600 (orb1526908) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB