P5CS/ALDH18A1 Antibody

• Catalog Number: orb623785
• Category: Antibodies
• Description: P5CS/ALDH18A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human P5CS/ALDH18A1 recombinant protein (Position: M1-A224).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 87 kDa
• UniProt ID: P54886
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Delta-1-pyrroline-5-carboxylate synthase; P5CS; Al
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-ALDH18A1 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-ALDH18A1 antibody.Lane 1:HeLa cell, Lane 2:MDA-MB-453 cell, Lane 3:Caco-2 cell, Lane 4:HEK293 cell, Lane 5:rat heart tissue, Lane 6:mouse skeletal muscle tissue.