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Catalog Number | orb764263 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to p53 (phospho-Thr18) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Thr18. AA range:1-50 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 46 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-TP53 antibody, anti-P53 antibody, anti-Cellul Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of rat-lung tissue. 1, p53 (phospho Thr18) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of rat-lung tissue. 1, p53 (phospho Thr18) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, p53 (phospho Thr18) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Western Blot analysis of various cells using Phospho-p53 (T18) Polyclonal Antibody diluted at 1:2000.
FC, IHC-P, WB | |
Bovine, Canine, Equine, Porcine, Sheep |
DOT, IHC-P, WB | |
Porcine, Rabbit | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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