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Catalog Number | orb6594 |
---|---|
Category | Tools |
Description | P53 (phospho-Ser15) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC-P, WB |
Predicted Reactivity | Bovine, Canine, Guinea pig, Porcine, Rabbit, Sheep |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from human P53 around the phosphorylation site of Ser15 (PL(p-S)QE) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1μg /test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 43 kDa |
Target | phospho-P53 (Ser15) |
UniProt ID | P04637 |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | p53 (Phospho-Ser15); P53 (phospho S15); P53 (phosp Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ICC analysis of A549 cell using phospho-P53 (Ser15) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
Western blot:Lane 1:Human A549 cell;Lane 2:Human MCF-7 cell;Primary:Anti-phospho-P53 (Ser15) at 1:1000 dilution.
IHC, IP, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, IP, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, IP, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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