p53 Antibody Cocktail

SKU: orb248577

Description

This antibody cocktail is specific for a 53kDa protein, which is identified as p53 suppressor gene product. The antibody cocktail reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. antibody to p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a (BP53-12) + Mouse IgG2b (DO-7)
• Clone No.: BP53-12 + DO-7
• Immunogen: Recombinant human wild-type p53 protein (BP53-12) & Recombinant human wild type p53 protein expressed in E. coli (DO-7) were used as the immunogen for this antibody cocktail.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of normal colon with p53 antibody cocktail (BP53-12 + DO-7).

Western blot testing of 293 cell lysate with p53 antibody cocktail (BP53-12 + DO-7).