P4HB Antibody

• Catalog Number: orb1255009
• Category: Antibodies
• Description: P4HB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: A synthetic peptide of human P4HB
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:200Flow: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 57kDa
• Target: P4HB
• UniProt ID: P07237
• Storage: Store at 4°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, pH 7.3.
• Alternative names: Protein disulfide isomerase family A member 1, DSI
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200Flow: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of COLO320 cells, using P4HB antibody (orb1255009). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.