P2Y8 antibody

• Catalog Number: orb315642
• Category: Antibodies
• Description: Rabbit polyclonal antibody to P2Y8
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human P2Y8. The exact sequence is proprietary.
• Dilution range: WB: 1-500:1000, IF/ICC: 1-100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: P2RY8
• Entrez: 286530
• UniProt ID: Q86VZ1
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti P2Y purinoceptor 8 antibody, anti P2Y8 antibo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of P2Y8 expression in Hela (A), mouse lung (B), mouse heart (C), mouse kidney (D), rat heart (E), rat kidney (F) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 50 kD)

Immunofluorescent analysis of P2Y8 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).