P2X5 antibody

• Catalog Number: orb667582
• Category: Antibodies
• Description: Rabbit polyclonal antibody to P2X5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human P2X5. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: P2RX5
• Entrez: 5026, 113995
• UniProt ID: P51578, Q93086
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-P2X5 antibody, anti-P2X purinoceptor 5 antibo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of P2X5 expression in K562 (A), mouse kidney (B), rat heart (C) whole cell lysates. (Predicted band size: 44; 47; 48 kD; Observed band size: 115 kD)

Immunofluorescent analysis of P2X5 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).