P2RX4/P2X4 Antibody

• Catalog Number: orb1539387
• Category: Antibodies
• Description: P2RX4/P2X4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Canine, Hamster, Human, Mouse, Rat
• Immunogen: Peptide with sequence C-ETDSVVSSVTTKAK, from the internal region of the protein sequence according to NP_035156.2.
• Concentration: 0.5 mg/ml
• Dilution range: IF (10 µg/ml), IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:16000), WB (0.3 - 1 µg/ml)
• Conjugation: Unconjugated
• Target: P2RX4 / P2X4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: P2RX4, ATP receptor, p2X purinoceptor 4, p2X4R, p2
• Note: For research use only
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 60kD band observed in Human Kidney and Liver lysates (calculated MW of 43.5kD according to NP_002551.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Brown et al, J Biol Chem. 2004 Sep 17;279(38):39485-94. PMID: 15262999). Recommended concentration: 1-3 ug/ml.
• Expiration Date: 12 months from date of receipt.

P2RX4 / P2X4 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).

P2RX4 / P2X4 antibody (0.3 µg/ml) staining of A549 cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.