p27 Kip1 (phospho-T198) antibody

• Catalog Number: orb213715
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CDKN1B
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T198 of human p27 Kip1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CDKN1B
• Entrez: 1027, 12576
• UniProt ID: P46414, P46527
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti KIP1 antibody, anti Cyclin-dependent kinase i
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of p27 Kip1 (Phospho-T198) expression in HEK293T (A), Hela (B), rat lung (C), rat kidney (D) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 27 kD)

Immunohistochemical analysis of p27 Kip1 (Phospho-T198) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.