p21 antibody

• Catalog Number: orb38089
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB3180
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, IF/ICC: 1:10-50, FACS: 1:10-50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 18119
• Target: CDKN1A
• UniProt ID: P38936
• NCBI: NP_000380.1, NP_510867.1, NP_001207706.1, NP_001207707.1
• Alternative names: Anti-"Cyclin-dependent kinase inhibitor 1 antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)

PubMed: 32050796

Applications

WB

Reactivity

Rat

Cui, Yuying et al. Decreased RNA?binding protein IGF2BP2 downregulates NT5DC2, which suppresses cell proliferation, and induces cell cycle arrest and apoptosis in diffuse large B?cell lymphoma cells by regulating the p53 signaling pathway Mol Med Rep, 26, 286 (2022)

PubMed: 35894142

Applications

WB

Reactivity

Human

Immunofluorescense analysis of 293 cell using p21 antibody (primary antibody dilution at: 1:10-50)

Immunohistochemical staining of paraffin embedded human cancer tissue using p21 antibody (primary antibody dilution at: 1:50-100)

Western blot analysis of T-47D cell lysatesusing p21 antibody (primary antibody dilution at: 1:1000)