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p19 Antibody (N-term)
Catalog Number: orb1927935
| Catalog Number | orb1927935 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB23860 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 17700 Da |
| Target | This p19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human p19. |
| UniProt ID | P55273 |
| NCBI | NP_001791.1, NP_524145.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Cyclin-dependent kinase 4 inhibitor D, p19-INK4d, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of p19 Antibody (N-term) in mouse testis tissue lysates (35 ug/lane). p19 (arrow) was detected using the purified Pab.
p19 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-p19 Antibody (N-term) at 1:2000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 18 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
p19 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the p19 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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