p16 antibody

SKU: orb2276359

Description

p16 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Pancreas: at least a moderate staining is expected in islets of Langerhans Negative Control: Pancreas: staining should be absent in acinar cells, Normal cervix uteri: p16 staining should be absent in epithelial cells (in some cases, few cells may show weak staining) Cellular Localization: Nuclear and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-016R
• Immunogen: Recombinant full-length human p16INK4a protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDK4 inhibitor p16 INK4; CDK4I; CDKN2A; Cell cycle negative regulator beta; CMM2; Cyclin dependent kinase 4 inhibitor A; Melanoma p16 inhibits CDK4; MLM; MTS1; Multiple tumor suppressor 1; p14; p16; p19; P19ARF; TP16

pancreas p16.

Serous ovarian carcinoma with variable intensity p16 immunostaining in all cells.

Squamous cell carcinoma of the cervix uteri HPV positive with strong p16 immunostaining.

Quick Database Links

UniProt

P42771