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| Catalog Number | orb1950661 |
|---|---|
| Category | Antibodies |
| Description | Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification | Affinity-chromatography |
| Immunogen | A synthesized peptide derived from human p150 CAF1 |
| UniProt ID | Q13111 |
| Tested applications | FC, ICC, IHC, IP, WB |
| Dilution range | WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:500 |
| Antibody Type | Recombinant Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CAF; CAF1; CAF1P150; CHAF1A; DCAF1; hp15; P150; |
| Note | For research use only |
![p150 CAF1 Rabbit mAb [KD Validated]](/images/pub/media/catalog/product/NewWebsite/18/orb1950661_1.png)
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
![p150 CAF1 Rabbit mAb [KD Validated]](/images/pub/media/catalog/product/NewWebsite/18/orb1950661_2.png)
Western blot analysis of p150 CAF1 expression in K562 cell lysate.
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