p120 Catenin Antibody / CTNND1 / Catenin delta 1

• Catalog Number: orb2636669
• Category: Antibodies
• Description: Alpha-catenin and beta-catenin bind to the intracellular domain of E-cadherin while p120 catenin (delta-1-catenin) binds E-cadherin at a juxta-membrane site. The complex stabilizes tight junctions. In the cell, delta-1-catenin localized to the E-cadherin/catenins cell adhesion complex, directly associates with cytoplasmic C-terminus of E-cadherin and may similarly interact with other cadherins.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CTNND1/4501
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 138-276 was used as the immunogen for the Delta 1 Catenin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Delta 1 Catenin antibody is available for research use only.
• UniProt ID: O60716
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Delta 1 Catenin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon tissue with Delta 1 Catenin antibody (clone CTNND1/4501) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative tissue control: IHC analysis of formalin-fixed, paraffin-embedded human brain stained with CTNND1/4501 at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.