You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1784576 |
---|---|
Category | Antibodies |
Description | P-Selectin/CD62P Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | ICC, IF, IHC, WB |
Reactivity | Human |
Immunogen | residues 42-771 (ECD) |
Concentration | 1000 µg/ml |
Dilution range | WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | P-Selectin/CD62P |
UniProt ID | P16109 |
Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
Alternative names | LECAM3; leukocyte-endothelial cell adhesion molecu Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human P-Selectin/CD62P by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, Platelets, Jurkat, HEL 92.1.7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human P-Selectin/CD62P by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of HEL 92.1.7 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human P-Selectin/CD62P (red) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:100. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor™ Plus 647 1:400. Counterstain: DAPI (blue).
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
Filter by Rating