P-Cadherin Antibody / CDH3

• Catalog Number: orb386133
• Category: Antibodies
• Description: Recognizes a protein of 116kDa, identified as P-Cadherin, or Cadherin 3 or CDH3. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congenital hypotrichosis with juvenile macular dystrophy.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6A9
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Membrane from trypsinized A431 cells was used as the immunogen for this P-Cadherin antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This P-Cadherin antibody is available for research use only.
• UniProt ID: P22223
• Storage: Store the P-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the P-Cadherin antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human A431 cell lysate using CDH3 antibody