Ovalbumin antibody

• Catalog Number: orb344565
• Category: Antibodies
• Description: Ovalbumin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Ovalbumin [Hen Egg White]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:25,000, IHC: 1:300 - 1,2000, IP: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Rabbit Anti-Ovalbumin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: P01012
• NCBI: P01012.2
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Ovalbumin Antibody, Egg albumin antibo
• Note: For research use only
• Application notes: Anti-Ovalbumin Antibody has been tested in Western Blot. Although not tested this product could be useful in assays such as ELISA, IHC, and IP. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ~1 ug Ovalbumin protein reduced (Lane1), 0.25 ug Ovalbumin protein reduced (Lane2), ~1 ug Ovalbumin protein non-reduced (Lane3), 0.25 ug Ovalbumin protein non-reduced (Lane4)4 using Ovalbumin antibody