Ovalbumin antibody

• Catalog Number: orb344564
• Category: Antibodies
• Description: Ovalbumin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Ovalbumin [Hen Egg White]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: 1:300 - 1:2,000, IP: 1:100, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Rabbit Anti-Ovalbumin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: P01012
• NCBI: P01012.2
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Ovalbumin Antibody, Egg albumin antibo
• Note: For research use only
• Application notes: Anti-Ovalbumin Antibody has been tested in Western Blot. Although not tested this product could be useful in assays such as ELISA, IHC and IP. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ~1 ug Ovalbumin protein reduced (Lane1), 0.25 ug Ovalbumin protein reduced (Lane2), ~1 ug Ovalbumin protein non-reduced (Lane3), 0.25 ug Ovalbumin protein non-reduced (Lane4) using Ovalbumin antibody