Osteocalcin Mouse Monoclonal Antibody

SKU: orb1817002

Description

Osteocalcin Mouse Monoclonal Antibody

Research Area

Osteogenesis

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-F=1:400-800, IF=1:100-500, IHC-P=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 2F10
• Immunogen: Recombinant human Osteocalcin protein (24-100/100aa)
• Target: BGLAP
• Molecular Weight: 11 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Disclaimer: For research use only

Alternative Names

OSTCN_HUMAN; BGLAP; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate protein; OC; OCN;

Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Osteocalcin) Monoclonal Antibody, Unconjugated (orb1817002) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Recombinant human Osteocalcin, C-hFc (HEK293), Primary: Anti-Osteocalcin (orb1817002) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 35.5 kDa.

Quick Database Links

Gene Symbol

BGLAP

UniProt

P02818