Osteocalcin Mouse Monoclonal Antibody

• Catalog Number: orb1817002
• Category: Antibodies
• Description: Osteocalcin Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2F10
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human Osteocalcin protein (24-100/100aa)
• Antibody Type: Primary Antibody
• Dilution range: WB=1:500-2000, IHC-F=1:400-800, IF=1:100-500, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 11 kDa
• Target: BGLAP
• UniProt ID: P02818
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Osteocalcin Propeptide; gamma-carboxyglutamic acid
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Osteocalcin) Monoclonal Antibody, Unconjugated (orb1817002) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Recombinant human Osteocalcin, C-hFc (HEK293), Primary: Anti-Osteocalcin (orb1817002) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 35.5 kDa.