OSBPL9 Antibody

• Catalog Number: orb1247162
• Category: Antibodies
• Description: OSBPL9 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EPLLKRLGAAKH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 150kDa and 19kDa in lysates of Human Brain, and Testis and 55kDa in lysates of Human Placenta and Testes after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the different calculated sizes of the six reported isoforms (a: 62.5kDa for NP_683702.1 and NP_683703.1; b: 63.9kDa for NP_683704.1; c: 70.2kDa for NP_683705.1; d: 81.8kDa for NP_683706.1; e: 83.2 kDa for NP_078862.2; f: 84.3kDa for NP_683707.1). All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Lung shows staining of alveolar macrophages. . Recommended concentration, 2-4ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: OSBPL9
• UniProt ID: Q96SU4
• NCBI: NP_683707.3, NP_078862.4, NP_683705.1, NP_683706.3, NP_683704.2, NP_683702.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: OSBPL9, ORP9, FLJ12492, FLJ14629, FLJ14801, FLJ320
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 150kDa and 19kDa in lysates of Human Brain, and Testis and 55kDa in lysates of Human Placenta and Testes after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the different calculated sizes of the six reported isoforms (a: 62.5kDa for NP_683702.1 and NP_683703.1; b: 63.9kDa for NP_683704.1; c: 70.2kDa for NP_683705.1; d: 81.8kDa for NP_683706.1; e: 83.2 kDa for NP_078862.2; f: 84.3kDa for NP_683707.1). All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Lung shows staining of alveolar macrophages. . Recommended concentration, 2-4ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247162 (2.5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.